Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Arabidopsis thaliana) | CTR1 | TAIR (https://www.arabidopsis.org/) | AT5G03730 | Receptor-coupled kinase involved in ethylene signaling |
| Gene (Arabidopsis thaliana) | BRXL2 | TAIR (https://www.arabidopsis.org/) | AT3G14000 | Polarity protein in the stomatal lineage |
| Gene (Arabidopsis thaliana) | BASL | TAIR (https://www.arabidopsis.org/) | AT5G60880 | Polarity protein in the stomatal lineage |
| Gene (Arabidopsis thaliana) | ATML1 | TAIR (https://www.arabidopsis.org/) | AT4G21750 | Homeobox transcription factor |
| Gene (Arabidopsis thaliana) | EIN2 | TAIR (https://www.arabidopsis.org/) | AT5G03280 | Component of ethylene signaling |
| Gene (Arabidopsis thaliana) | EIN3 | TAIR (https://www.arabidopsis.org/) | AT3G20770 | Transcriptional factor in ethylene signaling |
| Gene (Arabidopsis thaliana) | EIL1 | TAIR (https://www.arabidopsis.org/) | AT2G27050 | Transcriptional factor in ethylene signaling |
| Gene (Arabidopsis thaliana) | EBF1 | TAIR (https://www.arabidopsis.org/) | AT2G25490 | F-box protein involved in ethylene signaling |
| Gene (Arabidopsis thaliana) | EBF2 | TAIR (https://www.arabidopsis.org/) | AT5G25350 | F-box protein involved in ethylene signaling |
| Gene (Arabidopsis thaliana) | HXK1 | TAIR (https://www.arabidopsis.org/) | AT4G29130 | Hexokinase in the glucose signaling network |
| Gene (Arabidopsis thaliana) | SPCH | TAIR (https://www.arabidopsis.org/) | AT5G53210 | bHLH transcription factor involved in stomatal development |
| Gene (Arabidopsis thaliana) | TMM | TAIR (https://www.arabidopsis.org/) | AT1G80080 | LRR receptor-like protein involved in stomatal development |
| Strain, strain background (Arabidopsis thaliana) | Col-0 | ABRC | CS28166 | Wild-type Arabidopsis ecotype used in this study |
| Strain, strain background (Arabidopsis thaliana) | Ler-0 | ABRC | CS20 | Wild-type Arabidopsis ecotype used in this study |
| Strain, strain background (Agrobacterium tumefaciens) | GV3101 | Other | Electrocompetent A. tumefaciens |
|
| Genetic reagent (Arabidopsis thaliana) | ctr1-1 | Kieber et al., 1993; DOI:10.1016/0092-8674(93)90119-b | ||
| Genetic reagent (Arabidopsis thaliana) | ctr1-btk | Ikeda et al., 2009; DOI:10.3389/fpls.2017.00475 | ||
| Genetic reagent (Arabidopsis thaliana) | ein3eil1ebf1ebf2 | Sugano et al., 2010; DOI: 10.1105/tpc.110.076588 | ||
| Genetic reagent (Arabidopsis thaliana) | ein2-5 | Alonso et al., 1999; DOI:10.1126/science.284.5423.2148 | ||
| Genetic reagent (Arabidopsis thaliana) | gin2 | Moore et al., 2003; DOI:10.1126/science.1080585 | ||
| Genetic reagent (Arabidopsis thaliana) | hxk1-3 | Huang et al., 2015; DOI:10.3389/fpls.2015.00851 | ||
| Genetic reagent (Arabidopsis thaliana) | EIN3ox | Chao et al., 1997; DOI:10.1016/s0092-8674(00)80300–1 | ||
| Genetic reagent (Arabidopsis thaliana) | ctr1G738R | This paper | A ctr1 (in Col-0,Arabidopsis thaliana) mutant harboring a point mutation; first introduced in Figure 1 and fully described in Figure 1—figure supplement 1; request to DCB Laboratory, Stanford, USA | |
| Genetic reagent (Arabidopsis thaliana) | pTMM::amiRNA-CTR1 | This paper | Transgenic line expressing an artificial microRNA (amiRNA) knockdown construct targeting CTR1 under the stomatal lineage-specific promoter TMM; first introduced in Figure 2; request to DCB Laboratory, Stanford, USA | |
| Genetic reagent (Arabidopsis thaliana) | pATML1::CTR1; ctr1G738R | This paper | Transgenic line expressing CTR1 under the epidermal-specific promoter ATLML1 in the ctr1G738R mutant background; first introduced in Figure 1—figure supplement 1; request to DCB Laboratory, Stanford, USA | |
| Genetic reagent (Arabidopsis thaliana) | pBRXL2::BRXL2-YFP; pATML1::mCherry-RCI2A | Rowe et al., 2019; DOI: 10.1101/614636 | ||
| Genetic reagent (Arabidopsis thaliana) | pBASL::YFP-BASL; pATML1::mCherry-RCI2A | Rowe et al., 2019; DOI: 10.1101/614636 | ||
| Genetic reagent (Arabidopsis thaliana) | pSPCH::gSPCH-YFP; spch3 | Lopez-Anido et al., 2020; DOI: 10.1101/2020.09.08.288498 | ||
| Chemical compound, drug | Propidium iodide | Thermo Fisher | Thermo Fisher: P3566 | Dye for staining intercellular space in Arabidopsis |
| Chemical compound, drug | AZD-8055 | Fisher Scientific | Fisher Scientific: 50-101-5840 | ATP-competitive inhibitor of TOR |
| Chemical compound, drug | 1-Aminocyclopropane-1-carboxylic acid | Sigma | Sigma: A3903-100MG | Ethylene precursor |
| Chemical compound, drug | Glucose | Sigma | Sigma: G7021-1KG | |
| Chemical compound, drug | 3-O-Methyl-D-glucopyranose | Sigma | Sigma: M4849-10G | Non-metabolizable sugar |
| Chemical compound, drug | Sucrose | Sigma | Sigma: S3929-1KG | |
| Chemical compound, drug | Fructose | Sigma | Sigma: 1040071000 | |
| Commercial assay or kit | RNeasy plant mini kit | QIAGEN | QIAGEN: 74104 | |
| Commercial assay or kit | iScript cDNA synthesis kit | Bio-Rad | Bio-Rad: 170-8891 | |
| Commercial assay or kit | Ssoadvanced Universal SYBR Green Supermix | Bio-Rad | Bio-Rad: 172-5274 | |
| Software, algorithm | Leica Application Suite X | Leica | Version: 3.5.2.18963 | |
| Software, algorithm | FIJI |
Schindelin et al., 2012; DOI:10.1038/nmeth.2019 |
Version: 2.0.0-rc-69/1.52 p | |
| Software, algorithm | POME | Gong et al., 2021; DOI:10.1111/nph.17165 | Version:1.0.0 | |
| Software, algorithm | R | https://www.R-project.org/ | Version: 4.0.1 | |
| Software, algorithm | TrackMate | Tinevez et al., 2017; DOI: 10.1016/j.ymeth.2016.09.016 | Version: 6.0.1 | |
| Software, algorithm | RStudio | https://rstudio.com | Version: 1.3.959 |