Figure 4. Sodium selenite supplementation decreases IGF-1 levels in female mice and results in beneficial plasma hormone and chemical changes typically associated with MR.

Longitudinal comparisons of the plasma concentrations of (A) IGF-1, (B) leptin, (C) FGF-21, (D) adiponectin, (E) glucose, and (F) insulin for control-fed (CF), methionine-restricted (MR), and sodium selenite-supplemented (CF-SS) female mice. Bars denote SEM. Statistically significant differences (as compared with the corresponding CF values) are indicated (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001). N = 4 for all groups.

Figure 4—figure supplement 1. Sodium selenite supplementation-mediated increases in circulating FGF-21 levels are age-dependent.

Comparisons over time of average values for (A) total body mass, (B) food consumption, and (C) food consumption normalized to total body mass for control-fed (CF; black circles) and sodium selenite-supplemented (CF-SS; blue triangles) adult male mice (8 months old). Also shown are the plasma concentrations of (D) FGF-21 and (E) adiponectin for control-fed and sodium selenite-supplemented male mice at the conclusion of the experiment (8 weeks). For all panels, bars denote SEM. For panel D, the difference depicted is statistically significant (****p<0.0001), whereas no change in FGF-21 levels was observed for young male mice (2 months old; see Figure 3). In addition, sodium selenite supplementation fails to produce a significant difference in the plasma levels of adiponectin in adult male mice (panel E). N = 3 for both groups.