Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Synaptopodin (Rabbit polyclonal) |
Synaptic Systems | Cat#: 163002 RRID:AB_887825 |
IF (‘1:1000’) EM (‘1:100’) |
| Antibody | Anti-NeuN (Rabbit polyclonal) |
Abcam | Cat#: ab104225 RRID:AB_10711153 |
IF (‘1:500’) |
| Antibody | Anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Goat polyclonal) |
Invitrogen | Cat#: A-11034, RRID:AB_2576217 | IF (‘1:1000’) |
| Antibody | Anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Goat polyclonal) |
Invitrogen | Cat#: A-32732, RRID:AB_2633281 | IF (‘1:1000’) |
| Antibody | Anti-Rabbit IgG Nanogold-Fab' (goat polyclonal) |
Nanoprobes |
Cat#: 2004 RRID:AB_2631182 |
EM (‘1:100’) |
| Biological sample (Homo sapiens), male and female | Sample | Biobank of the Department for Neurosurgery at the Faculty of Medicine, University of Freiburg, AZ 472/15_160880 | Approval of the Local Ethics Committee, University of Freiburg, AZ 593/19 | |
| Chemical compound, drug | DAPI (1 mg/ml in water) | Thermo Scientific | Cat#: 62248 | IF and post hoc labeling (‘1:5000’) |
| Chemical compound, drug | Pierce16% Formaldehyde (w/v), methanol-free | Thermo Scientific | Cat#: 28906 | Final concentration: (4% in PBS) |
| Chemical compound, drug | Glutardialdehyd | Carl Roth | Cat#: 4157.2 | Final concentration: 2.5% (TEM) and 0.1% (Immunogold) |
| Chemical compound, drug | All-trans retinoic acid | Sigma–Aldrich | Cat#: R2625 | Final concentration: 1 µM |
| Chemical compound, drug | Anisomycin | Abcam | Cat#: ab120495 | Final concentration: 10 µM |
| Chemical compound, drug | Actinomycin D | Sigma–Aldrich | Cat#: A9415 | Final concentration: 5 µg/ml |
| Commercial assay, kit | HQ Silver Enhancement Kit | Nanoprobes | Cat#: 2012 | |
| Genetic reagent Mus musculus, male |
B6.129-Synpotm1Mndl/Dllr; Synpo−/− |
Vlachos et al., 2013
PMID:23630268 |
MGI: 6423115 | Obtained from Deller Lab (Frankfurt) |
| Genetic reagent Mus musculus, male |
B6.Cg-Synpotm1MndlTg(Thy1-Synpo/GFP)1Dllr/Dllr; Thy1-GFP/Synpo+/− x Synpo−/− |
Vlachos et al., 2013
PMID:23630268 |
MGI: 6423116 | Obtained from Deller Lab (Frankfurt) |
| Peptide, recombinant protein | Streptavidin, Alexa Fluor 488-Conjugate | Invitrogen | Cat#: S32354 RRID:AB_2315383 |
Post hoc labeling (‘1:1000’) |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | Clampfit (pClamp software package) |
Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | ImageJ | RRID:SCR_003070 | ||
| Software, algorithm | Photoshop | Adobe | RRID:SCR_014199 | |
| Strain, strain background Mus musculus | C57BL/6J; Synpo+/+ | Jackson Laboratory | RRID: IMSR_JAX:000664 |