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. 2021 Mar 11;10:e63512. doi: 10.7554/eLife.63512

Figure 3. CBP/p300 histone acetyl transferase inhibitor A-485 causes H3 hypoacetylation and decreased early viral gene expression.

Figure 3.

(a) Western blot for H3K18ac, H3K9ac, and total H3 in human bronchial-tracheal epithelial cells treated with control vehicle DMSO (dimethyl sulfoxide) or 10 µM A-485 after 2, 3, or 4 hr. (b) H3K18ac, H3K27ac, and H3K9ac ChIP-seq at early viral promoters in cells treated with control vehicle DMSO (dimethyl sulfoxide) or 10 µM A-485 for 2 hr. (c) qRT-PCR for E2early, E3, and E4 pre-mRNA transcripts in cells treated with DMSO (dimethyl sulfoxide) or 10 µM A-485 for 2 hr. Averages and standard deviations shown for three successive experiments. Paired t-test, *p<0.05 between cells treated with DMSO (dimethyl sulfoxide) and A-485. ‘ns’ indicates no significant difference between cells treated with DMSO (dimethyl sulfoxide) and A-485.

Figure 3—source data 1. Average fold change in pause index for E2early, E3, and E4 in cells expressing wt E1A compared to DM E1A.