FIGURE 3:

Density distributions of bipolar cells, cones and horizontal cells. (a) All-type Bipolar cells (All-BCs) were identified by labelling with visual system homeobox 2 (Chx10) antibodies. Somas counted were tightly clustered and brightly labelled in the inner nuclear layer. (b) Cones were identified by labelling cone arrestin (CAR) with antibodies. Cones were marked at the start of the inner segment, below the synaptic terminal. (c) Horizonal cells were identified by labelling with calbindin (Calb) antibodies. Somas were brightly labelled within the inner nuclear layer, proximal to the outer nuclear layer. (d) Density by region of All-BCs, cones, and horizontal cells, respectively. All-BC density does not vary by region. Regional All-BC densities are as follows: Dorsal: 26,815 ± 2,336 cells/mm2; Ventral: 27,247±1,837 cells/mm2; Nasal: 27,242 ± 1,001 cells/mm2; Temporal: 26,208 ± 1,869 cells/mm2 (mean ± SD; N= 2 retinas, n = 43 fields, n = 128,898 cells). (e) Cone density does not vary by region. Regional cone densities are as follows: Dorsal: 14,912 ± 1,587 cells/mm2; Ventral: 14,423 ± 1,913 cells/mm2; Nasal: 13,788 ± 1,247 cells/mm2; Temporal: 14,710 ± 1,539 cells/mm2 (N = 2 retinas, n = 38 fields, n = 54,541 cells). (f) Horizontal cell density change is most prominent along the dorsal-ventral axis (p= 2.23 E-8). Horizontal cell densities are as follows: Dorsal: 1,295 ± 247 cells/mm2; Ventral: 1,664 ± 294 cells/mm2; Nasal: 1,442 ± 147 cells/mm2; Temporal: 1,466 ± 165 cells/mm2. (N = 5 retinas, n = 113 fields, n = 26,651 cells). When comparing between regions, an ANOVA with Tukey’s post-hoc indicated significant difference between regions. Difference between regions are denoted with asterisks (*). (g) BC density has negligible correlation with eccentricity (distance from the optic nerve) (ρ = −0.26771; R² = 0.0717). (h) Cone density has negligible correlation with eccentricity (ρ = −0.21338; R² = 0.0455). (i) HC density has a weak negative correlation with eccentricity (ρ = −0.43039; R² = 0.1852). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.