FIGURE 4:

Type 1a BC mapping and spatial heterogeneity. (a) Type 1a BC (BC1as) were identified by using the MitoP line expressing cyan fluorescent protein (CFP) in BC1as and nGnG amacrine cells. CFP fluorescence was amplified using anti-GFP. BC1as were identified as CFP⁺, Ppp1r17⁻ in conjunction with having a dendrite process extending into the OPL. (b) Density contour map of BC1as centred around the optic nerve (N = 4 retinas, n = 91 fields, n = 28,588 cells). (c) Density of BC1as by region. Dorsal: 1,904 ± 801 cells/mm²; Ventral: 2,471 ± 329 cells/mm²; Nasal/Temporal: 2,349 ± 473 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc showed significant difference between regions. Difference between regions are denoted with asterisks (*). (d) Voronoi tessellation of BC1as at low, medium and high densities. (e) Negligible positive correlation with eccentricity (distance from the optic nerve. (e) and BC1a spatial heterogeneity (ρ = 0.2537; R² = 0.0644). (f) BC1a spatial heterogeneity has weak negative correlation with cell density (ρ = −0.4591; R² = 0.2107). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.