FIGURE 5:

Type 1b BC density mapping and spatial heterogeneity. (a) Type 1b BCs (BC1bs) were identified by using the MitoP line expressing cyan fluorescent protein (CFP) in BC1bs and nGnG amacrine cells. CFP fluorescence was amplified using anti-GFP. (i) BC1bs were identified as CFP⁺, Ppp1r17⁻ in conjunction with lacking a dendrite process projecting into the OPL and absent in the INL (ii-iii) residing mostly in the ACL (denoted with arrows), whereas BC1as had a dendrite process (denoted with arrows). (b) Density contour map BC1bs centred around the optic nerve (N = 4 retinas, n = 91 fields, n = 13,018 cells). (c) Density of BC1bs by region. Dorsal: 839 ± 340 cells/mm²; Ventral: 1099 ± 167 cells/mm²; Nasal/Temporal: 1077 ± 214 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc test showed significant difference between regions. Difference between regions are denoted with asterisks (*). (d) Voronoi tessellation of BC1bs at low, medium and high densities. (e) Negligible positive correlation with eccentricity (distance from the optic nerve) and spatial heterogeneity (ρ = 0.07911; R² = 0.00277). (f) As BC1b cell spatial heterogeneity has negligible negative correlation with cell density (ρ = −0.14677; R² = 0.0321). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.