FIGURE 6:

Type 2 BC density mapping and spatial heterogeneity. (a) Type 2 BCs (BC2s) were identified by labelling with Synaptotagmin 2 (Syt2) antibodies. Faint somas with brightly labelled dendrite branches were identified as BC2s. (b) Density contour map of BC2s centred around the optic nerve (N = 8 retinas, n = 169 fields, n = 74,264 cells). (c) Density of BC2s by region are as follows: Dorsal: 2,200 ± 619 cells/mm²; Ventral: 2,723 ± 378 cells/mm²; Nasal: 2,592 ± 455 cells/mm²; Temporal: 2,626 ± 511 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc test showed significant difference between regions. Significance is denoted with asterisks (*). (d) Voronoi tessellation of BC2s at low, medium and high densities. (e) Negligible positive correlation with eccentricity (distance from the optic nerve) and Type 2 BC spatial heterogeneity (ρ = 0.18898; R² = 0.0066). (f) BC2 cell spatial heterogeneity has a weak negative correlation with cell density (ρ = −0.31994; R² = 0.0425). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.