FIGURE 7:

Type 3a BC density mapping and spatial heterogeneity. (a) Type 3a BC (BC3as) were identified by labelling with hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4) antibodies. Brightly labelled somas with dendritic process were denoted as BC3as. Faintly labelled cells without dendritic process extending into the outer plexiform layer were denoted as amacrine cells. (b) Density contour map of BC3as centred around the optic nerve (N= 8 retinas, n = 181 fields, n = 66,774 cells). (c) Density of type BC3as by region. Dorsal: 1976 ± 668 cells/mm²; Ventral: 2,958 ± 349 cells/mm²; Nasal: 2,579 ± 455 cells/mm²; Temporal: 2,661 ± 533 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc test showed significant difference between regions. Significance is denoted with asterisks (*). (d) Voronoi tessellation of BC3as at low, medium and high densities. (e) Weak positive correlation with eccentricity (distance from the optic nerve) and BC3a spatial heterogeneity (ρ = 0.44308; R² = 0.2602). (f) BC3a cell spatial heterogeneity has a moderate negative correlation with cell density (ρ = −0.69318; R² = 0.397). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.