FIGURE 8:

Type 3b BC density mapping and spatial heterogeneity. (a) Type 3b BC (BC3bs) were identified by labelling with protein kinase CAMP-dependent type II regulatory subunit beta (PKARIIβ) antibodies. (b) Density contour map of BC3bs centred around the optic nerve (N= 8 retinas, n = 174 fields, n = 69,464 cells). (c) Density of BC3bs by region. Dorsal: 3,335 ± 734 cells/mm²; Ventral: 3,785 ± 488 cells/mm²; Nasal: 3,579 ± 569 cells/mm²; Temporal: 3,700 ± 591 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc test showed significant difference between regions. Significance is denoted with asterisks (*). (d) Voronoi tessellation of BC3bs at low, medium and high densities. (e) Negligible negative correlation with eccentricity (distance from the optic nerve) and BC3b spatial heterogeneity (ρ = −0.01487; R²= 6E-05). (f) BC3b cell spatial heterogeneity has a negligible negative correlation with cell density (ρ = −0.12835; R² = 0.0231). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.