FIGURE 9:

Type 4 BC density mapping and spatial heterogeneity. (a) Type 4 BCs (BC4s) were identified by labelling with calsenilin (Csen) antibodies. Somas labelled with calsenilin that did not have a dendritic process extending into the outer plexiform layer were denoted as amacrine cells. (b) Density contour map of BC4s centred around the optic nerve (N= 6 retinas, n = 134 fields, n = 48,339 cells). (c) Density of BC4 by region. Dorsal: 2,051 ± 857 cells/mm²; Ventral: 2,529 ± 477 cells/mm²; Nasal: 2,370 ± 673 cells/mm²; Temporal: 2,503 ± 623 cells/mm². When comparing between regions, an ANOVA with Tukey’s post-hoc test showed significant difference between regions. Significance is denoted with asterisks (*). (d) Voronoi tessellation of BC4s at low, medium and high densities. (e) Negligible positive correlation with eccentricity (distance from the optic nerve) and BC4 spatial heterogeneity (ρ= 0.28006; R²=0.1008). (f) BC4 cell spatial heterogeneity has a weak negative correlation with cell density (ρ= −0.47239; R²=0.1997). For the full list of p-values, see Table S1. p ≤ 0.05; p ≤ 0.01; p ≤ 0.001 is indicated by *, **, and *** respectively. Pearson’s coefficient is denoted as ρ.