FIGURE 2.

LJA5 axons selectively target sensory regions. (a) A representative injection site of AAV‐DIO‐synaptophysin‐mCherry into LJA5 of this Pdyn‐GFP mouse shows that mCherry expression is limited to green fluorescent protein (GFP)‐expressing neurons (scale bar is 50 μm). (b) Synaptophysin‐mCherry densely labels presynaptic boutons throughout lamina I and the lateral spinal nucleus of the entire spinal cord, including upper thoracic (middle) and sacral (right) levels, and in the lateral parabrachial nucleus (left). LJA5 source neurons in the injection site are marked by a red arrow in the ventral brainstem of the first section (scale bars are 50 μm). (c) LJA5 boutons (mCherry+) more densely target lamina I (marked by CGRP peripheral axons) than lamina II (marked by IB4) (scale bar is 100 μm)