Figure 2. Developmental overlap between Brn3c, Brn3a and Brn3b in the mouse retina.

(a–l) Alkaline phosphatase (AP) staining in flat-mounted retinas (a–f) or cryostat retinal vertical sections (g–l) from adult mice of indicated genotypes. (m–r) Immunostaining of cryostat retinal vertical sections from adult mice of indicated genotypes with antibodies against AP, Brn3c and Brn3a (m–o) or AP, Brn3c and Brn3b (p–r). Individual panels for m–r show AP (first), Brn3c (second), Brn3a or Brn3b (third) and merge channel. All panels are counterstained with DAPI. Genotypes of samples are: CAG:Dre; Brn3c^CKOCre/WT; ROSA26^AP/WT (a, g, m, p); Brn3c^Cre/WT; Brn3a^CKOAP/WT (b, h, n, q); Brn3c^Cre/WT; Brn3b^CKOAP/WT (c, i, o, r); CAG:Dre; ROSA26^AP/WT (d, j); Brn3c^WT/WT; Brn3a^CKOAP/WT (e, k); Brn3c^WT/WT; Brn3b^CKOAP/WT (f, l). (m1–o1) Box plot distributions of AP⁺, Brn3a⁺, and Brn3c⁺ cells as percentages of DAPI cells in the ganglion cell layer (GCL), corresponding to figures m–o. (p1–r1) Box plot distributions of AP⁺, Brn3b⁺, and Brn3c⁺ cells as percentages of DAPI cells in the GCL, corresponding to figures p–r. Horizontal red lines are medians, blue squares are interquartile intervals, and whiskers ranges of observations. Red crosses are outliers. For AP histochemical stains, retinas from three mice of each genotype were analyzed. Immunofluorescence data for each genotype and staining condition was collected from 6 – 8 images from three distinct animals. Total number of counted cells are indicated for each staining and genotype, next to the panel label. Counted cells for each genotype and antibody combination are provided in Supplementary Table 2. Scale bars: (a–f) = 500 μm (g–l, m–r) = 25 μm.