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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1926–1953. doi: 10.1002/cne.25065

Figure 5. Morphological categorization of bistratified RGC types in Brn3cCre/WT adult mouse retina.

Figure 5

(a–d) AAV-GFP immunofluorescent 40x images of representative cells in the flat-mounted retina. Scale bar = 50 μm. (a1–d1) Corresponding reconstructions of cells a–d in en face (top) and vertical (bottom) projections. Axons are green, vitreal (ON) dendrites are blue, and scleral (OFF) dendrites are red. Yellow, cyan, and magenta depict recursive dendrites, which travel backwards from one plexus into the other. (e–j) Scatter grams of morphological parameters for bistratified RGC types. Measurements and landmarks are as in Figure 4. (e) Outer distance vs. inner distance relative to the normalized inner plexiform layer. For each cell the “ON” arbor is represented by a circle, and the “OFF” arbor by a square. (f) Inner dendritic arbor area vs. inner distance of the “ON” arbor. (g) Inner arbor area vs. distance of cell body from optic disk. (h) Outer area vs. inner area. (i) Outer area vs. inner distance of the “OFF” arbor. (j) Outer area vs. cell distance from optic disk. Cell type assignments are indicated by colors, and provided in the legend. The measurements of example cells a–d are indicated by arrows.