Figure 7. Physiological Classification of Brn3c^Cre RGCs.

(a) Bar plots of cell counts labeled in the Brn3c^Cre/WT; Rosa26^tdTomato line (black bars) and in adult Brn3c^Cre/WT mice injected with AAV2-CAG-FLEX-EGFP (green outlines). The upper plot shows the raw cell counts, while the lower plot normalizes the cell counts by type density in the Eyewire RGC catalogue (http://museum.eyewire.org/). (b–f) Examples of visual stimulus response properties for four identified types. Panels b, d, f, h illustrate peristimulus histograms for 1 second (yellow square) presentations of light spot of 200 μm in diameter from darkness to 200 R*/rod/s. x axis is time in seconds and y axis represents spiking frequency in Hz, scale bar is 200 Hz. Panels c, e, g, i represent responses (measured in spike counts) to characteristic stimuli for each cell type (see also Methods, and Schwartz et al in preparation http://rgctypes.org/). The four illustrated cell types are: (b) ON OS with flashed orientation bar response (c); (d) OFFhOS with drifting gratings response (e); (f) ON-OFF DS with moving bar response (g); and (h) ON tr. MeRF with receptive field size and surround suppression measured using light spots of increasing size (i). Abbreviations: OS, orientation selective; hOS, hortizontal OS; vOS, vertical OS; tr., transient; MeRF, medium receptive field; SmRF, small receptive field; sus., sustained; LED, local edge detector; sSbC, sustained suppressed-by-contrast; bSbC, bursty suppressed-by-contrast; EW, Eyewire; HD1, high definition type 1; HD1, high definition type 2; UHD, ultra high definition. See also Supplementary table 3.