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. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: Eur J Med Chem. 2021 Feb 3;215:113252. doi: 10.1016/j.ejmech.2021.113252

Table 1.

RIPK2 and ALK2 kinases, and NOD2 cell (HEKBlue) signaling inhibitory activities of 14 and 15 – 20.

graphic file with name nihms-1669616-t0010.jpg
Compound R1 Kinase IC50 (μM) RIPK2/NOD2
Cell Assay
IC50 (μM)
RIPK2 ALK2
14 2,6-di-Cl 0.013 ± 0.004 0.021 ± 0.016 0.037 ± 0.004
15 2,4-di-Cl 0.016 ± 0.0036 NI 0.16 ± 0.024
16 2-Cl 0.017 ± 0.008 0.062 ± 0.080 0.004 ± 0.0005
17 4-Cl 0.013 ± 0.0006 2.940 ± 1.633 0.95 ± 0.35
18 4-t-Bu 0.105 ± 0.047 NI 1.98 ± 1.73
19 4-OH 0.025 ± 0.014 0.059 ± 0.065 0.005 ± 0.002
20 4-OMe 0.021 ± 0.0016 NI 0.51 ± 0.12

NI: No inhibition observed at 100 μM; IC50 values are shown as the mean of two or more determinations ± standard deviation.