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. Author manuscript; available in PMC: 2022 Mar 30.
Published in final edited form as: Circulation. 2021 Jan 21;143(13):1317–1330. doi: 10.1161/CIRCULATIONAHA.120.051517

Figure 3. Tissue stiffening and concomitant increase in cardiomyocyte size are prevented with 5-HT2B blockade.

Figure 3.

Atomic force microscopy (AFM) was employed to analyze scar and BZ stiffness after MI. Each heart was arrested in diastole via submersion in 3M potassium chloride upon dissection. A, Each row illustrates the distribution of stiffness values within an individual mouse with representative histogram in the middle. B, Mean tissue stiffness of scar and BZ (N=3–9). C, qPCR analysis of Nppb, the gene encoding the heart failure marker natriuretic peptide B (N=4). D-E, Wheat germ agglutinin (WGA) staining of short-axis cardiomyocytes reveals increased cross-sectional area in control treated animals after MI compared to sham operation is prevented with 5-HT2B antagonism (N=3–12). B-C, E, Mean ± SEM, *P<0.05, **P<0.01 (B,E) 2-way ANOVA and Holm-Sidak post hoc test or (C) 2-tailed Student t test.