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. Author manuscript; available in PMC: 2022 Mar 30.
Published in final edited form as: Circulation. 2021 Jan 21;143(13):1317–1330. doi: 10.1161/CIRCULATIONAHA.120.051517

Figure 6. Htr2b deletion impairs proliferative and remodeling capabilities of cardiac fibroblasts (CFs).

Figure 6.

A, Volcano plot highlighting differentially regulated genes in PDGFRα+ cells isolated from Htr2bfl/flPostnMCM/+ (N=4) compared to Htr2bfl/fl animals (N=2). B, Increased expression of Dnajb in 5-HT2B-knockout CFs (N=3). C, Phosphorylated Src is decreased in isolated CFs lacking 5-HT2B (normalized to total Src and loading control α-tubulin, N=4). D, Periostin immunostaining reveals a decrease in myofibroblast presence in damaged tissue one week after MI (N=4). E, 5-HT2B-knockout CFs exhibit decreased proliferation measured by BrdU incorporation (N=3). F, 5-HT2B-knockout CFs are less migratory than WT counterparts (N=3). G, Free-floating collagen gel contraction assay reveals a decrease in collagen matrix remodeling by 5-HT2B-knockout CFs (N=6). B-G, Mean ± SEM, *P<0.05, following (B-E) 2-tailed Student t test or (F,G) 2-way ANOVA and Holm-Sidak post hoc test.