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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 13. mWAKE+ cells in the TMN.

FIGURE 13

Representative images of mWake expression in 2 areas of the TMN are shown (the perifornicular region and the medial 3v region). (a) and (g) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b) and (h) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c) and (i) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. The arrows in (a) indicate the dorsal (d) and medial (m) directions and also apply to (b) and (c). (d-f, j-l) mWAKE+ neurons in the TMN are distinct from histaminergic neurons. tdTomato fluorescence (red) (d and j), anti-HDC (green) (e and k), and merged images (f and l) from mWake(Cre/+) are shown. All scale bars denote 500 μm and apply to other panels across a row. Abbreviations: 3v, third ventricle; fx, fornix; HDC, histidine decarboxylase; ot, optic tract; TMN, tuberomammillary nucleus.