FIGURE 15. mWake expression in the LH.
(a-c) Representative images of mWake expression in the LH area. (a) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. Dashed lines in (a-c) demarcate the edge of the optic tract along the ventral edge of the hypothalamus. The arrows in (a) indicate the dorsal (d) and medial (m) directions. Scale bar denotes 500 μm and applies to other panels across a row. Abbreviations: fx, fornix; LH, lateral hypothalamus; ot, optic tract.
