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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 16. mWake expression in subcortical regions.

FIGURE 16

Representative images of mWake expression in subcortical areas. Images of a ZI (a-c), the nDB (c-f), a CPu (g-i), a SPFp (j-l), and the SFO (m-o) are shown. In (a-c), the dashed line outlines the ZI. In (d-f), thicker dashed lines mark the perimeter of the nDB, and the thinner dashed line encircles the ICjM. Dashed lines outline the SPFp (j-l) and SFO (m-o), respectively. Asterisks label tdTomato+ processes in (c) and (f). “+” symbols in (m-o) indicate mWAKE+ ependymal cells. (a), (d), (g), (j), and (m) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b), (e), (h), (k), and (n) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c), (f), (i), (l), and (o) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. The black boxes in (h) and (n) depict the location of the inset in the lower right corner, where the scale bars denote 50 μm. Scale bars in (a), (d), (g), (j), and (m) denote 500 μm and apply to other main panels across a row. Arrows in (a), (g), and (j) indicate the dorsal (d) and medial (m) directions, and apply to (b) and (c), (h) and (i), and (k) and (l), respectively. Abbreviations: 3v, third ventricle; cc, corpus collosum; CPu, caudate putamen; ICjM, Islands of Calleja insula magna; nDB, nucleus of the diagonal band; ot, optic tract; SFO, subfornicular organ; SPFp, subparafascicular nucleus parvicellular part; ZI, zona incerta.