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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 17. Limbic system centers with mWAKE+ cells.

FIGURE 17

Representative images of mWake expression in brain regions of the limbic system. (a) BNST, LSc, and surrounding area. (b-d) Images of the LSc, with the thicker dashed line outlining the boundary of the LSc, and the thinner dashed line marking the limits of the MS. (e-g) Images of a BNST, encircled by a dashed line. (h-j) Images of an amygdala area, with sub-regions LA, CeA, and BMA denoted by dashed outlines. (k-m) Images of the ICjM, encircled by the thinner dashed line, while the thicker dashed line marks the medial limits of the NAc. (n-p) Images of multiple ICjs, with each individual island outlined by thin dashed lines. (a), (b), (e), (h), (k) and (n) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (c), (f), (i), (l), and (o) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (d), (g), (j), (m), and (p) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. Asterisks in (d), (g), and (j) mark the presence of tdTomato+ fibers and processes. Scale bar in (a) denotes 200 μm. Scale bars in (b), (e), (h), (k), and (n) denote 500 μm and apply to other panels across a row. Arrows in (e), (h), and (n) indicate the dorsal (d) and medial (m) directions, and apply to (f) and (g), (i) and (j), and (o) and (p), respectively. Abbreviations: ac, anterior commissure; BMA, basomedial amygdala; BNST, bed nucleus of the stria terminalis; cc, corpus collosum; CeA, central amygdala; ICj, islands of Calleja; ICjM, islands of Calleja, insula magna; LA, lateral amygdala; LSc, lateral septal center; lv, lateral ventricle; MS, medial septal nucleus; NAc, nucleus accumbens.