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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 18. mWake expression in the midbrain.

FIGURE 18

(a-c) mWAKE-V5 expression in the periqueductal gray at different A-P coordinates relative to Bregma: −4.0 mm (a), −4.5 mm (b), and −4.8 mm (c). (d-f) vlPAG denoted by thicker dashed lines, and DR outlined by a thinner dashed line. (g-i) mWAKE+ neurons in the vlPAG/DR are distinct from serotonergic neurons. (j-l) SC, outlined by the dashed line. (m-o) mWAKE expression in the PBG. (a-c) anti-V5 IF staining (green) in mWake(V5/+) mice. (d), (j), (m) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (e), (k), (n) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (f), (l), (o) Endogenous tdTomato fluorescence (red) in a mWake(Cre/+) mouse with DAPI. tdTomato fluorescence (red) (g), anti-TPH2 (green) (h), and merged images (i) from mWake(Cre/+) mice are shown. Scale bar in (a) denotes 200 μm and applies to (b) and (c). All other scale bars denote 500 μm and apply to other panels across a row. Arrows in (m) indicate the dorsal (d) and medial (m) directions, and apply to (n) and (o). Asterisks in (l) label tdTomato+ fibers. Abbreviations: aq, aqueduct; DR, dorsal raphe; PBG, parabigeminal nucleus; SC, superior colliculus; TPH2, tryptophan hydroxylase 2; vlPAG, ventrolateral periaqueductal gray.