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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 19. mWake expression in the pons.

FIGURE 19

(a) The LC and surrounding region. (b-d) LC area encircled by a dashed line. (e) mWAKE+ neurons are near the LC. (f-h) mWAKE+ neurons near the LC are distinct from noradrenergic neurons. (i-k) PB, with the entire area encircled by the thicker dashed line, and the PBdl, PBe, and PBm subregions outlined by thin dashed lines. (a), (b), and (i) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (c, j) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (d, k) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. (e) anti-TH (green), tdTomato fluorescence (red), and DAPI in mWake(Cre/+) mice. tdTomato fluorescence (red) (f), anti-TH (green) (g), and merged images (h) from mWake(Cre/+) mice are shown. Scale bars in (a) and (e) denote 200 μm. Scale bars in (b), (f), and (i) denote 500 μm and apply to other panels across a row. Arrows in (b) and (i) indicate the dorsal (d) and medial (m) directions, and apply to (e) and (f), and (j) and (k), respectively. Asterisks in (d) label tdTomato+ fibers. Abbreviations: 4v, fourth ventricle; aq, aqueduct; DR, dorsal raphe; LC, locus coeruleus; PBdl, dorsolateral parabrachial nucleus; PBe, external parabrachial nucleus; PBm, medial parabrachial nucleus; scp, superior cerebellar peduncle; TH, tyrosine hydroxylase.