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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 20. Sensory-related regions containing mWake expression.

FIGURE 20

Representative images of mWake expression in sensory-related areas of the brainstem. Images of a CN (a-c), IC (d-f), SuVe (g-i), and MVe (j-l) are shown. In (a-c), the dashed line outlines the CN. In (d-f), the thicker dashed line outlines the IC, which is divided into medial and lateral portions by the thinner dashed line. In (g-i), the dashed line encircles the SuVe. (a), (d), (g), and (j) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b), (e), (h), and (k) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c), (f), (i), and (l) endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. The black boxes in (b), (h), and (k) highlight areas of ISH signal. Asterisks in (f) mark tdTomato+ fibers. Scale bars in (a), (d), (g), and (j) denote 500 μm and apply to other panels across a row. Arrows in (a), (d), (g) and (j) indicate the dorsal (d) and medial (m) directions, and apply to other panels across a row. Abbreviations: 4v, fourth ventricle; CN, cochlear nucleus; IC, inferior colliculus; MVe, medial vestibular nucleus; scp, superior cerebellar peduncle; SuVe, superior vestibular nucleus.