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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 21. mWAKE+ expression in the paleocortex.

FIGURE 21

Representative images of mWake expression in 2 structures of the paleocortex. (a-c) OB, with dashed line indicating the outer limits of the granule cell layer. (d-f) Pir, with dashed lines separating layers I, II, and II. (a) and (d) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b) and (e) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c) and (f) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. In (c) and (f), asterisks indicate tdTomato+ processes. Black box in (b) depicts the location of the inset in the lower right corner, where the scale bar denotes 50 μm. Scale bars in (a) and (d) denote 500 μm, and apply to other main panels across a row. Arrows in (d) indicate the dorsal (d) and medial (m) directions, and apply to (e) and (f). Abbreviations: OB, olfactory bulb; Pir, piriform cortex.