FIGURE 22. mWake expression within the neocortex.

Representative images of mWake expression in the neocortex. (a-c) Somatosensory area of the cortex, with dashed lines separating the SSp, SSb, and SSs, and solid lines delineating layers I, II/III, IV, V, and VI. (d) Higher-magnification image of the SSb area containing tdTomato⁺ cell bodies and processes, with cortical layers labeled. (e-g) AudC and TeA, separated by dashed lines with cortical layers I, II/III, IV, V, and VI separated by solid lines. (h-j) Bilateral ACC, with a dashed line separating the dorsal (d) and ventral (v) portions. (k) A representative higher-magnification image of the tdTomato⁺ projections from the approximate region marked by the asterisks in (j). (l) Higher-magnification image of tdTomato fluorescence in the cc. (a), (e), and (h) Anti-V5 IF staining (green) with DAPI in mWake^(V5/+) mice. (b), (f), and (i) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c), (d), (g), (j), (k), and (l) Endogenous tdTomato fluorescence (red) in mWake^(Cre/+) mice with DAPI. In (c), (d), (j), and (l), asterisks indicate tdTomato⁺ processes. The black box in (b) depicts the location of the inset in the upper right corner, where the scale bar denotes 100 μm. The black box in (i) highlights ISH signal along the dorsal-ventral axis of the ACC. Scale bar in (a) denotes 1 mm, and applies to (b) and (c). Scale bars in (d) and (l) denote 300 μm. Scale bars in (e) and (h) denote 500 μm and apply to (f) and (g), and (i) and (j), respectively. Scale bar in (k) denotes 100 μm. Arrows in (a) and (e) indicate the dorsal (d) and medial (m) directions, and apply to (b), (c), (f) and (g). Abbreviations: ACC, anterior cingulate cortex; AudC, auditory cortex; cc, corpus callosum; SSb, somatosensory cortex barrel region; SSp, primary somatosensory cortex; SSs, supplemental somatosensory cortex; TeA, temporal cortex area.