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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 23. Ependymal cells express mWake.

FIGURE 23

Representative images of mWake expression in ependymal cells. Images of the 3v (a-c), 4v (d-f), and one lv (g-i) are shown. (a), (d), and (g) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b), (e), (h) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c), (f), and (i) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. In all images, “+” indicates mWAKE+ ependymal cells. Black boxes in (b), (e), and (h) indicate the location of the inset in the lower right corner, where the scale bars denote 30 μm. Scale bars in (a), (d), and (g) denote 300 μm and apply to (b) and (c), (e) and (f), and (h) and (i), respectively. Arrows in (g) indicate dorsal (d) and medial (m) directions, and apply to (h) and (i). Abbreviations: 3v, third ventricle; 4v, fourth ventricle; cc, corpus collosum; LSc, lateral septal center; lv, lateral ventricle.