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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 24. mWake expression in additional regions.

FIGURE 24

Representative images of mWake expression in 2 additional structures. Images of mWAKE expression in a supra-hypothalamic region likely Xi (a-c) and a round mid-cortical cluster, likely in the PRh (d-f), encircled by a dashed line. In addition, broader view images of mWAKE expression are shown for the supra-hypothalamic region (g) and the mid-cortical cluster (h). In (g), dashed lines indicate the Xi and the surrounding Re, and mWAKE expression in the aZI and anterolateral hypothalamus is also noted. In (h), dashed lines outline the PRh and encircle the DEn. (a), (d), (g), and (h) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (b) and (e) chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (c) and (f) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. The scale bars in (a) and (d) denote 500 μm, and apply to other panels across a row. Scale bars in (g) and (h) denote 200 μm. Arrows in (d) indicate the dorsal (d) and medial (m) directions, and apply to (e) and (f). Abbreviations: 3v, third ventricle; aZI, anterior zona incerta; DEn, dorsal endopiriform nucleus; f, fornix; LA, lateral amygdala; PRh, perirhinal area of the cortex; PVN, paraventricular nucleus; Re, thalamic reuniens nucleus; Xi, xiphoid nucleus.