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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 3. Overview of mWAKE expression in the murine brain.

FIGURE 3.

Schematic using selected Paxinos and Franklin coronal plates illustrating areas of significant mWAKE expression, as determined by RNAscope in situ hybridization, mWAKE-V5 immunostaining, and endogenous tdTomato fluorescence in mWake(Cre/+) mice. Areas of patchy or sparse mWAKE expression are denoted by stippled fill, whereas dense expression is indicated by denser fill. A-P coordinates relative to Bregma for the sections are listed above. Abbreviations: 3v-ependymal, ependymal cells of 3rd ventricle; ACc, anterior cingulate cortex; AudC, auditory cortex; BMA, basomedial amygdala; BNST, bed nucleus of the stria terminalis; cc, corpus callosum; CeA, central amygdala; CN, cochlear nucleus; CPu, caudate putamen; DMH, dorsomedial hypothalamus; ICj, islands of Calleja; ICjM, islands of Calleja insula magna; LA, lateral amygdala; LC, locus coeruleus; LH, lateral hypothalamus; LS, lateral septum; MVe, medial vestibular nucleus; nDB, nucleus of the diagonal band; OB(gc), olfactory bulb granule cell layer; PB, parabrachial nucleus; PBG, parabigeminal nucleus; Pir, piriform cortex; Re, thalamic reuniens nucleus; SC, superior colliculus; SCN, suprachiasmatic nucleus; SFO, subfornical organ; SSc, somatosensory cortex; SuVe, superior vestibular nucleus; TMN, tuberomammillary nucleus; vlPAG, ventrolateral periaqueductal gray; VMH, ventromedial hypothalamus; vmPOA, ventromedial preoptic area; and ZI, zona incerta.