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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Nov 10;529(8):1954–1987. doi: 10.1002/cne.25066

FIGURE 8. mWAKE+ cells in the DMH.

FIGURE 8

Representative images of mWake expression in the DMH are shown. (a-c) Anti-V5 IF staining (green) with DAPI in mWake(V5/+) mice. (d) Chromogenic RNAscope ISH for mWake mRNA (red) with hematoxylin counterstain (purple) in wild-type (WT) mice. (e) Endogenous tdTomato fluorescence (red) in mWake(Cre/+) mice with DAPI. (a, b) DMH, posterior DMH, and surrounding region. Dashed lines outline the DMH and VMH in (a) and the posterior DMH in (b). In (c-e), the dashed outline denotes the boundary of the DMH. Scale bar denotes 200 μm in (a) and (b). In (c), the scale bar is equivalent to 500 μm, and the arrows indicate dorsal (d) and medial (m) directions, and both also apply to (d) and (e). Abbreviations: 3v, third ventricle; DMH, dorsomedial hypothalamus; fx, fornix; VMH, ventromedial hypothalamus.