Fig. 3.

In vitro gene expression with estradiol treatment in osteoblast/osteocyte mesenchymal stem cell line. a FGF23 expression was significantly increased with 0.1mM estradiol treatment as compared to vehicle (p=0.033). Addition of 1,25-vitamin D did not produce any additional effect. b Esr1 expression was no different with any concentration of estradiol of 1,25-vitamin D treatment. c Esr2 expression was significantly increased with 0.1mM estradiol (p=0.001) and 0.1mM + 1,25-vitamin D treatment (p=0.002). Data plotted as mean and standard deviation with point plots representing individual animal data (n=3 for all treatments). *statistically different from all other groups (p<0.05).