FIGURE 2 |.

(A) Illustration on the preparation method of NC probes for immunoassay. (B) The fluorescence and absorption spectra of the NC probes. The inset images present a representative TEM image of the prepared NC probes and their emission under UV light. (C) Representative fluorescence decay of the NC probes diluted in pure water—the NC probes emit fluorescence after laser-off. The inset presents the background for pure water. (D) Calibration curve of the NC probes in water (with 5% BSA mimicking a high autofluorescent sample matrix) measured using a nongated microplate reader. (E) Calibration curve of the NC probes in water (with 5% BSA) measured using the time-gated platform.