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. Author manuscript; available in PMC: 2021 Jul 1.

Published in final edited form as: Front Phys. 2021 Jan 27;8:625424.

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FIGURE 3 | — (A) Dilution optimization of antibody-coated NC probes for immunoassay. (B) Optimal selection of immunoaffinity separation solutions for immunoassay (all separation solutions contain 0.1% BSA and 1% SDS, and the optimization experiments were done with measurements using a standard non-time-gated microplate reader). (C) Calibration curves of immunoassays measured using a standard nongated microplate reader. (D) Calibration curves of immunoassays measured using the time-gated platform.