Figure 6. IRF-1 recruits immune cells via CXCL10/CXCR3 axis in murine HCC tumor.

A) qRT-PCR analysis for CXCL10 and CXCR3 mRNA expression in murine HCC tumors overexpressing IRF-1 (IRF-1) vs. negative control (NC). (B, C) The statistical summary of CXCR3 positive cell rate in CD8+T cells, NK cells and NKT cells in single cell suspension from same volume of tumors expressing IRF-1 vs. NC. Representative images of FACS assay for CXCR3 positive cell rate in CD8+T cells (D), NK cells (E) and NKT cells (F) in tumors expressing IRF-1 vs. NC. Each data point (A, B & C) represents one mouse (n=4–5). Data represent mean ± SD, *p<0.05, **p<0.01.