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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 16;166:90–103. doi: 10.1016/j.freeradbiomed.2021.01.054

Figure 1. SIRT6 protein levels and gene expression are not altered by age or OA in primary human chondrocytes.

Figure 1.

(A) Unstimulated primary human articular chondrocytes isolated from young (av age 29±7.7 yrs), old (av age 66±3.9 yrs) and OA (av age 61±11.8 yrs) cartilage were subjected to immunoblotting for total SIRT6. Representative immunoblots from young, old and OA samples are shown. (B) Densitometric analysis showing chondrocyte SIRT6 protein levels from young (n=14), old (n=15) and OA (n=14) cartilage tissue. SIRT6 was normalized to β-actin as a loading control. Quantified data is mean±SD showing individual data points from independent samples. Two-way ANOVA was used to test for differences between groups (NS = non-significant). (C) Basal SIRT6 gene expression measured by RT-qPCR in unstimulated articular chondrocytes derived from normal cartilage from adults aged 17-72 yrs. (n=57). The lowest value was set to 1 and SIRT6 levels were normalized to YWHAZ as a housekeeping control. R2 and p-values from linear regression analysis are presented.