Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 16;166:90–103. doi: 10.1016/j.freeradbiomed.2021.01.054

Figure 3. Effect of adenoviral overexpression of SIRT6 on basal antioxidant protein levels.

Figure 3.

(A) Human articular chondrocytes were transduced with an empty adenoviral vector control (ad-Null) or an adenoviral vector encoding SIRT6 (ad-SIRT6) for 48 hours. Basal protein levels of Prx2, Prx3, Prx4, Prx6, Trx1, Trx2, HO-1, NQO1 and Nrf2 were assessed by immunoblotting. (B) Densitometric analysis from Prx2 (n=8), Prx3 (n=8), Prx4 (n=7), Prx6 (n=7), Nrf2 (n=15), Trx1 (n=8), Trx2 (n=8), HO-1 (n=7), NQO1 (n=7), and Nrf2 (n=15) immunoblots. Representative immunoblots are shown. Total protein levels were normalized to β-tubulin as a loading control. SIRT6 immunoblots confirmed SIRT6 overexpression. Quantified data is mean±SD showing individual data points from independent samples. Paired t-tests were used to test for statistical differences between group