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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 16;166:90–103. doi: 10.1016/j.freeradbiomed.2021.01.054

Figure 5. Effect of adenoviral overexpression of SIRT6 on basal TXNIP, Prx1 and Srx levels in OA human articular chondrocytes.

Figure 5.

(A) Human OA articular chondrocytes were transduced with ad-Null or ad-SIRT6 for 48 hours. Basal protein levels of TXNIP, Prx1 and Srx from total cell lysates were assessed by immunoblotting. (B) Densitometric analysis from TXNIP (n=11), Prx1 (n=11) and Srx (n=9) immunoblots. Total protein levels were normalized to β-tubulin as a loading control. In all cases, representative immunoblots are shown. SIRT6 immunoblots confirmed SIRT6 overexpression. Paired t-tests were used to test for statistical differences between groups.