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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 16;166:90–103. doi: 10.1016/j.freeradbiomed.2021.01.054

Figure 6. Effect of Sirt6 deficiency on chondrocyte TXNIP and Prx1 protein levels.

Figure 6.

(A) Paired femoral caps from Aggrecan-CreERT2; Sirt6fl/fl (Sirt6 loss) or Sirt6fl/fl (Sirt6 intact) mice were treated with 4-Hydroxytamoxifen to activate Cre-mediated recombination ex vivo. Cell lysates were prepared and basal protein levels of TXNIP and Prx1 were assessed by immunoblotting. (B) Densitometric analysis from basal TXNIP and Prx1 immunoblots (n=3). In all cases, total protein levels were normalized to β-tubulin as a loading control. SIRT6 immunoblots confirmed Sirt6 loss. Unpaired t-test was used to test for statistical differences between groups.