Figure 4.

Mechanical properties of right and left tibiae; standard-CON (n=10), standard-RAL (n=12), thermoneutral-CON (n=11), thermoneutral-RAL (n=12). A) Average force-displacement plot for standard temperature, showing stronger loaded bones (mean +/− SEM). B) The strength disparity continued at the thermoneutral temperature as quantified by C) the ultimate force. D) Average stress-strain plots for mice housed at standard temperature show how tissue-level properties increase with loading and RAL, E) with similar results at thermoneutral temperature. F) Bone toughness increases with loading and RAL treatment. **** p<0.0001, ** p=0.0092, * p≤0.05.