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. Author manuscript; available in PMC: 2022 May 28.
Published in final edited form as: Cancer Lett. 2021 Mar 4;506:83–94. doi: 10.1016/j.canlet.2021.02.021

Fig. 3. FGL2 induces CXCL7 expression in macrophages.

Fig. 3.

(A), Experimental scheme for RNA-seq analysis of BMDMs treated with FGL2hi conditioned media (CM) vs FGL2KO CM. (B), Volcano plots showing DEGs in BMDMs exposed to FGL2hi CM as compared with FGL2KO CM. (C), KEGG pathway enrichment analysis of DEGs selected in the heatmap in (C). (D), Waterfall plot of DEGs. The criteria were: (1) genes in the heatmap and (2) paired FC > 2 or < -2. (E), Protein levels of CXCL7 in medium of cultured BMDMs. Data were analyzed by 1-way ANOVA (n = 3). (F), Protein levels of CXCL7 in brain lysates at day 7 after tumor implantation. Data are presented as the mean ± SD and were analyzed by t test (n = 3). (G), Representative immunofluorescent staining of CXCL7 (in red) and IBA1 (in green) in human glioblastoma tissues. Area indicated with rectangle is enlarged and shown on the right side. Scale bar, 200 μm. White arrows: co-immunofluorescent staining. Representative data from 2–3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.