Fig. 3.

Reconstructed μCT images were analyzed and several parameters were examined within the fracture callus region, including: mineralized callus volume (BV/TV) (A), trabecular number (Tb.N) (B), trabecular thickness (Tb.Th) (C), trabecular separation (Tb.Sp) (D), and RUST scoring (E) for each group. For RUST scoring, each cortex of the fracture callus as observed on the reconstructed images (orthogonal views) were given a score of 1 (callus absent), 2 (callus present, incomplete bridging), or 3 (callus present, complete bridging), and the scores for all 4 cortices were combined to provide a final score ranging from 4 (not healed) to 12 (maximally healed). Data are expressed as mean ± SD (n=7-11/group). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001.