Key Resources Table
| REAGENT or RESOURCES | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| Tamoxifen | Sigma | T5648 |
| Humilin R | Eli Lilly and Company | NDC 0002-8215-91 (HI-210) |
| Isoflurane | Patterson Veterinary | NDC 14043-704-5 |
| Recombinant human bone morphogenetic protein-2 (rhBMP-2) | Medtronic Sofamor Danek Inc | 7510903 |
| Sodium chloride 0.9% in aqueous solution Normal saline solution, sterile | Aqualite Systems | NDC 0409-5168-38 |
| Rodent Diet with 10kcal% fat (LOW FAT) | Research Diets | D12450H |
| Rodent Diet with 45kcal% fat HIGH FAT) | Research Diets | D12451 |
| Eye Lubricant Major® LubriFresh™ P.M. 3.5 Gram Eye Ointment | Major Pharmaceuticals | 904648838 |
| Reagent Alcohol | Decon Laboratories, Inc | 2705SG |
| Collagenase type 2 | Worthington Biochemical Corporation | NC9522060 |
| Dynabeads™ Biotin Binder | Thermo Fisher Scientific | 11047 |
| Endothelial Cell Growth Medium 2 | PromoCell | C-22011 |
| Endothelial Cell Medium | ScienCell | 1001 |
| Fetal bovine serum | ScienCell | 0025 |
| Endothelial Cell Growth Supplement | ScienCell | 1052 |
| Penicillin/Streptomycin Solution | ScienCell | 0503 |
| Penicillin-Streptomycin-Glutamine | Thermo Fisher Scientific | 10378016 |
| Fibronectin | Gibco | 33016015 |
| Richard-Allan Scientific™ Neutral Buffered Formalin (10%) | Thermo Fisher Scientific | 5700TS |
| Phosphate-buffered saline | IBI Scientific | IB70166 |
| Probumin® BSA Products | Sigma | 82-002-4 |
| DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride) | Thermo Fisher Scientific | D1306 |
| Corning™ Matrigel™ Membrane Matrix | Corning® | 354234 |
| RNeasy Mini Kit | QIAGEN | 74106 |
| Transcriptor First Strand cDNA Synthesis Kit | Roche | 4897030001 |
| Power SYBR™ Green PCR Master Mix | Thermo Fisher Scientific | 4367659 |
| Corning® Collagen 1, Rat Tail, 100mg | Corning® | 354236 |
| Povidone-Iodine Solution USP, 10% (w/v), 1% (w/v) Available Iodine, for Laboratory Use, Ricca Chemical | Purdue Products, LB | NDC 67618-150-01 |
| Antibodies/Primers | ||
| MS CD31 Biotin MAB, 0.5mg MEC 1 | BD Biosciences | 553371 |
| Anti-CD31/PECAM-1, DyLight 488, Clone: MEC 7.46, antibody | Novus Biologicals™ | NB1001642G |
| Materials | ||
| 3-0 polyglycolic acid suture | ETHICON | J215H |
| EZ clips non-sterile 7-mm | Braintree Scientific | RF7CS |
| Fisherbrand™ Sterile Cell Strainer (70 μm) | Thermo Fisher Scientific | 22-363-548 |
| Corning® BioCoat™ Collagen l-coated Plates | Corning® | 356400 |
| Corning® 96-well Clear Polystyrene Microplates | Corning® | 3596 |
| RCM6 Resorbable Collagen Membrane | ACE Surgical Supply Co., Inc. | 509-3000 |
| MS CD31 Biotin MAB, 0.5mg MEC 1 | BD Biosciences | 553371 |
| Anti-CD31/PECAM-1, DyLight 488, Clone: MEC 7.46, antibody | Novus Biologicals™ | NB1001642G |
| Instruments/Software | ||
| EchoMRI™-100H Body Composition Analyzer | EchoMRI, LLC | 100 H |
| 200 Two Speed Rotary Tool | DREMEL | 200 Series |
| Invitrogen DynaMag-2 Magnet | Thermo Fisher Scientific | 12321D |
| EVOS® FL Cell Imaging System | Thermo Fisher Scientific | EVOS® FL |
| IncuCyte ZOOM® Live-Cell Analysis System | ESSEN BIOSCIENCE | ZOOM |
| IncuCyte™ Scratch Wound Cell Migration Software | ESSEN BIOSCIENCE | 9600-0012 |
| IncuCyte® Cell Migration Kit | ESSEN BIOSCIENCE | 4493 |
| CFX96 Touch Real-Time PCR Detection System | Bio-Rad | 1855196 |