Table 1.
Sample-based frequency of phosphorylation at HCN1 phosphosites in rat and human brain tissues.
| HCN1 phosphosites | ||||
|---|---|---|---|---|
| HCN1 phosphosite (rat - Q9JKB0) | ratio (%) of samples found phosphorylated (rat, n = 42) | HCN1 phosphosite (human - O60741) | ratio (%) of samples found phosphorylated (human, n = 10) | species in which previously reported |
| T39 | 42/42 (100.0%) | T41 | 8/10 (80.0%) | rat/human |
| S53 | 5/42 (11.9%) | S56 | (0%) | |
| S69 | 40/42 (95.2%) | #(G80) | # | rat |
| T88 | 2/42 (4.8%) | T99 | (0%) | human |
| S105 | 17/42 (40.5) | S116 | (0%) | human |
| T461 | 9/42 (21.4%) | T472 | (0%) | |
| Y502 | 6/42 (14.3%) | Y513 | 1/10 (10.0%) | human |
| S588 | 6/42 (14.3%) | S599 | (0%) | |
| #(A635) | # | T646 | 1/10 (10.0%) | |
| S791 | 38/42 (90.5%) | S770 | 5/10 (50.0%) | |
| T792 | 33/42 (78.6%) | T771 | 3/10 (30.0%) | |
| S867 | 42/42 (100.0%) | S846 | 5/10 (50.0%) | rat/human |
| S868 | 7/42 (16.7%) | S847 | 1/10 (10.0%) | rat/human |
| S891 | 30/42 (71.4%) | S871 | 3/10 (30.0%) | |
| #(P892) | # | S872 | 4/10 (40.0%) | |
| S893 | 3/42 (7.1%) | S873 | 4/10 (40.0%) | |
Analogous HCN1 phosphosites between rat and human are displayed on the same line. (#) indicates corresponding amino acid residues where phosphorylation is not possible. Shown for each phosphosite is the ratio (%) of all samples analyzed where phosphorylation was detected, with boldface lettering indicating phosphorylation that occurred in at least 50% of samples within a species. Known HCN1 phosphosites in rats and humans are reported in the Eukaryotic Phosphorylation Site Database (Lin et al., 2020). Novel HCN1 phosphosites in which phosphorylation was detected by mass spectrometry are labeled in red.