Table 2.
Sample-based frequency of phosphorylation at HCN2 phosphosites in rat and human brain tissues.
| HCN2 phosphosites | ||||
|---|---|---|---|---|
| HCN2 phosphosite (rat - Q9JKA9) | ratio (%) of samples found phosphorylated (rat n = 19) | HCN2 phosphosite (human - Q9UL51) | ratio (%) of samples found phosphorylated (human, n = 10) | species in which previously reported |
| S67 | 3/19 (15.8%) | S80 | 3/10 (30.0%) | rat/human |
| S68 | 1/19 (5.3%) | S81 | (0%) | rat/human |
| T70 | (0%) | T87 | 2/10 (20.0%) | rat/human |
| #(G77) | # | S97 | 1/10 (10.0%) | |
| S90 | 18/19 (94.7%) | S110 | 4/10 (40.0%) | rat |
| S109 | 3/19 (15.8%) | S132 | 4/10 (40.0%) | human |
| S119 | 19/19 (100%) | S146 | 8/10 (80.0%) | rat/human |
| S743 | 18/19 (94.7%) | S771 | 6/10 (60.0%) | rat/human |
| S750 | 19/19 (100%) | S779 | 7/10 (70.0%) | rat/human |
| S756 | 19/19 (100%) | #(A785) | # | rat |
| S757 | 19/19 (100%) | S786 | 6/10 (60.0%) | rat/human |
| S764 | 9/19 (47.4%) | S793 | 8/10 (80.0%) | rat/human |
| Y766 | (0%) | Y795 | 6/10 (60.0%) | rat/human |
| S771 | 13/19 (68.4%) | #(A801) | # | rat |
| T827 | 1/19 (5.3%) | #(A853) | # | |
| S834 | 15/19 (79.0%) | S860 | 3/10 (30.0%) | rat/human |
| S840 | 15/19 (79.0%) | S866 | 6/10 (60.0%) | rat/human |
| S842 | 19/19 (100%) | S868 | 8/10 (80.0%) | rat/human |
| S847 | 9/19 (47.4%) | #(G873) | # | rat |
Analogous HCN2 phosphosites between rat and human are displayed on the same line. (#) indicates analogous amino acid residues where phosphorylation is not possible. Shown for each phosphosite is the ratio (%) of all samples analyzed where phosphorylation was detected, with boldface lettering indicating phosphorylation that occurred in at least 50% of samples within a species. Known HCN2 phosphosites in rat and humans are reported in the Eukaryotic Phosphorylation Site Database (Lin et al., 2020). Novel HCN2 phosphosites in which phosphorylation was detected by mass spectrometry are labeled in red.