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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 17;166:73–89. doi: 10.1016/j.freeradbiomed.2021.02.006

Figure 1.

Figure 1.

KYC Decreased BPD in Hyperoxic Pups. (A) Representative immunohistochemistry images of lung sections from PBS- (Left panel) and KYC-treated, hyperoxic neonatal rat pups. Images show that KYC decreased MPO+ myeloid cell counts relative to MPO+ cell counts in lungs of hyperoxic pups (n=9). The bar chart shows the mean ± SD of MPO+ cell counts in lung sections from hyperoxic pups treated with PBS and KYC, respectively. (B) Immunoblots show that lung lysates from KYC-treated hyperoxic pups contain less MPO protein than lung lysates from PBS-treated hyperoxic pups (n=9). The bar chart shows the mean ± SD of the band densities for MPO relative to actin in lung lysates from hyperoxic pups treated with PBS and KYC. (C) Immunoblots show that lung lysates from KYC-treated hyperoxic pups have less Cl-Tyr immunoreactive proteins than lung lysates from PBS-treated hyperoxic pups (n=9). Quantification of immunoreactive Cl-Try protein densities was by scanning the entire lane and normalizing the integrated scanned profile to actin, an internal control (Hyperoxia = O2 > 90%; * = p<0.05).