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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 17;166:73–89. doi: 10.1016/j.freeradbiomed.2021.02.006

Figure 2.

Figure 2.

KYC Prevented Alveolar Simplification in Lungs of Hyperoxic Pups. (A) Representative H&E images of lung sections showing alveolar and vascular simplification resulting from chronic hyperoxia that were reversed with KYC treatment. RAC are increased in lung sections from KYC-treated hyperoxic pups (left, n=9) compared to RAC levels in lung sections from PBS-treated hyperoxic pups (right, n=12). The bar chart shows the mean ± SD for RAC from lung sections from hyperoxic pups treated with PBS and KYC. (B) Representative H&E images of lung sections from hyperoxic pups treated with PBS (left) vs. KYC (right). Arrows indicate lungs structures counted as secondary septa. Bar charts summarize secondary septation data and show that KYC treatment of hyperoxic pups increased secondary septation (right, n=9) compared to secondary septation in PBS-treated hyperoxic pups (left, n=12). The bar chart shows the mean ± SD for secondary septa in lung sections from hyperoxic pups treated with PBS and KYC. (C) Representative H&E images showing capillary structures in lung sections from hyperoxic pups treated with PBS (left) vs. KYC (right). Arrows indicate lung structures counted as capillaries. The bar chart shows capillary counts are increased in KYC-treated hyperoxic pups (right, n=9) compared with counts in PBS-treated hyperoxic pups (left, n=12). (D) Representative H&E images of lung sections from PBS-treated (left) and KYC-treated (KYC) hyperoxic pups. The bar chart shows MLI are decreased in lung sections in KYC-treated hyperoxic pups (right, n=9) compared to MLI in lungs of PBS-treated hyperoxic neonatal rat pups (left, n=12). (E) Representative immunoblots for CD31 relative to actin, an internal loading control from lung lysates prepared from KYC-treated hyperoxic pups (right, n=9) and PBS-treated hyperoxic pups. (left, n-9). The bar chart shows the mean ± SD of relative differences in CD31 band densities normalized to actin in lung lysates from PBS and KYC treated hyperoxic pups. (Hyperoxia = O2 > 90%, * = p<0.05).