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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2021 Feb 17;166:73–89. doi: 10.1016/j.freeradbiomed.2021.02.006

Figure 8.

Figure 8.

MPO Oxidizes KYC to a KYC Thiyl Radical That Thiylates HMGB1. (A) ESR spectrum of KYC thiyl S•-DMPO generated by an MPO reaction mixture containing MPO (120 nM), H2O2 (50 μM) and KYC (30 μM). (B) Streptavidin affinity-blot of KYC thiylated HMGB1 and MPO in a split sample, one half treated neat and other half treated with DTT (100 mM) to reduce disulfide bonds. The affinity-blot shows that DTT reduced B-KYC-thiylated HMGB1 and B-KYC-thiylated MPO fluorescent band densities, confirming that the bond between KYC and HMGB1 and MPO were both disulfides.