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. 2021 Mar 30;11:7134. doi: 10.1038/s41598-021-86647-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CLSM of biofilm formation on the titanium surface. High-intensity fluorescence was seen in the TSB, 1/8MIC SH, and 1/4 MIC SH groups, and low-intensity fluorescence was seen in the 1/2MIC SH, 1MIC SH, and vancomycin groups. (a) Control group. (b) 1/8MIC SH group. (c) 1/4MIC SH group. (d) 1/2MIC SH group. (e) 1MIC SH group. (f) Vancomycin group. Magnification, ×400. Scale bars, 50 µm.