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. 2021 Mar 30;11:7197. doi: 10.1038/s41598-021-85477-y

Figure 1.

Figure 1

The absence of Myo1g affects adhesion and rolling of activated B lymphocytes on high endothelial venules. (a) Representative diagram of the different HEVs and (b) measurement of the diameter of venules (from the narrow IV to wide I) in the WT host mice's inguinal lymph nodes. LPS + IL-4 activated B cells (stained with Hoechst 33342) from WT or Myo1g−/− mice were directly injected into the carotid artery of a WT mouse. One hour previously, the inguinal lymph node was injected with 100 μl CXCL13 (25 ng/ml). Measurements of B cell flux (frequency of leukocytes that pass through the postcapillary venules) for 1 min (c) and 5 min (d). (e) Quantification of the number of activated B cells from WT and Myo1g−/− mice, performing slow rolling (frequency of leukocytes with a rolling velocity less than 5 μm/s) in the different venules (IV to I) of an inguinal lymph node of a host WT mouse. (f) Measurements of rolling velocities (traveling time from venules IV to I) of activated B cells from WT and Myo1g-/- mice in the WT inguinal lymph node. (g) Quantification of adherent B cells in the different postcapillary venules after 45 min. (h) Quantification of extravasated B cells at 45 min. n = 3. Data are presented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.